Collectively, snRNA-seq analysis refined the distinct roles carried out by obesity-induced macrophage subsets: lipid handing and lysosomal activation, observed in TREM2 + CD11C+ macrophages (Mod4-enriched) and phagocytosis and anti-inflammatory restoration evident in LYVE1 + CD206+ macrophages (Mod2-enriched) (Fig. 4D–G). This evidence concerns the gene LYVE1 and obesity due to melanocortin 4 receptor deficiency.