To identify genes upregulated in obese adipose tissue, we analyzed three publicly available bulk RNA-seq datasets (Fig. 1A, B): gonadal white adipose tissue (GWAT) of normal chow diet (NCD) and high-fat diet (HFD) fed mice (GSE182930)23, GWAT of wild type and leptin-deficient (ob/ob) mice (GSE150102)24, and human abdominal subcutaneous WAT of lean subjects and patients with obesity (GSE59034)25. The gene discussed is LEP; the disease is obesity due to melanocortin 4 receptor deficiency.