Here we identify the interaction of Pol δ and other replisome components with the AAA+ ATPases SPATA5 and SPATA5L1, which are mutated in the human neurodevelopmental syndromes EHLMRS (Epilepsy, Hearing Loss, and Mental Retardation Syndrome) and NEDHLS (Neurodevelopmental Disorder with Hearing Loss and Spasticity)13–18. This evidence concerns the gene AFG2B and neurodevelopmental disorder.