GRN and cancer: To further address the question which cells PGRN was derived from for CD8+ T cell exclusion, either cancer cells or macrophages, we, respectively, generated monoclonal PY8119 cell line without PGRN (Grn−/− PY8119KO), in which the endogenous Grn gene was deleted via CRISPR/Cas9 and chimeric Grn-deficient mice in the BM compartment (Grn−/− BMKO mice).