Besides T cells activation and proliferation, we also detected the direct effect of PVRIG mAbs on cytotoxic T cells, consistent with other data, IBI352g4a failed to elicit any cytotoxic mediators, such as IFN-r, Granzyme B and Perforin at various dose from 0.01 to 10 ug/mL at different incubation time from 24 to 120 h (Fig. S2d), and induced direct T cells-mediated tumor killing effect (Fig. S2e). The gene discussed is PRF1; the disease is neoplasm.