Colorectal cancer cells were cultivated using three different conditions: naive macrophages-conditioned medium (M0-CM), M0 macrophages treated with IL-4-conditioned medium (IL-4-CM), and M0 macrophages treated with both IL-4 and 23-HBA-conditioned medium (IL-4+23-HBA-CM). The gene discussed is IL4; the disease is colorectal cancer.