TNF and Hyperglycemia: In line with our in vitro model of hyperglycemia, at the end of differentiation, CAD-T2DM-derived monocytes still showed, as their precursors, high expression levels of CDKi p21 and p27 genes, and release, after LPS stimulation, of proinflammatory IL6 and TNFα cytokines (Fig. 7E–G).