To test if the role of FGF2 could shift from promoting proliferation to inducing a stem-like state in FGFR1 amplified cells, we next investigated the effect of FGF2 on cancer stemness-like traits using FACS analysis for ALDH and CD44 (two known markers of breast cancer stemness) [52]. This evidence concerns the gene FGFR1 and breast carcinoma.