TNF and hepatocellular carcinoma: The best model of each 3-,4-, and 5-biomarker panels were confirmed by qRT-PCR, and the 3-marker panel of TNF-α, RNASE2, and MAP3K7 found to have the best performance on experimental data, suggesting that it has the potential to be used as a non-invasive diagnostic panel with high accuracy for detecting HCC compared to healthy controls and CHB patients.