Our multiplex immunofluorescence (Multiplex-IF) analysis revealed that HOXB3 expression in IPMN tissues decreases as the dysplasia grade increases, and it is entirely absent in high-grade dysplasia IPMN, irrespective of the IPMN subtype (Mean: Normal Duct, 0.2%; LGD, 91.3%; BR, 43.9%; Gastric, 1.0%; Intestinal, 0.8%; PB, 0.6%). This evidence concerns the gene HOXB3 and pancreatic intraductal papillary-mucinous neoplasm.