Our chemogenetic activation experiments indicate that the adult spinal Glra3-Cre populations have an acute sensory role as its activation evoked spontaneous sensory behaviors, such as licking/biting, stomping, and guarding of the affected dermatome, whereas silencing decreased compound 48/80- and chloroquine-induced itch, indicative of a pro-pruritic role. Here, GLRA3 is linked to Pruritus.