HOTAIR and PVT1 showed elevated levels in PC tissues and saliva compared to healthy controls and benign tumors. Their discriminatory power for PC detection was evaluated, demonstrating good sensitivity and specificity, outperforming serum CA19-9. Salivary levels decreased after curative pancreatectomy. The findings suggest HOTAIR and PVT1 as potential non-invasive biomarkers for PC detection. This evidence concerns the gene PVT1 and pachyonychia congenita.