For example, cluster 10 expressed similar levels of Cst3 and Igfbp2 to other astrocyte-clusters, but also had globally distinguishing genes including Vim (vimentin), an intermediate filament that is canonically expressed in cerebral astrocytes, Slc6a13 (GAT-2), a major GABA transporter, and Gjb2 (Cx26), a CO2-sensitive gap junction for which defects have been implicated in congenital sensorineural hearing loss including KID syndrome as well as glial dysregulation31. Here, IGFBP2 is linked to Congenital sensorineural hearing impairment.