GJB2 and Congenital sensorineural hearing impairment: For example, cluster 10 expressed similar levels of Cst3 and Igfbp2 to other astrocyte-clusters, but also had globally distinguishing genes including Vim (vimentin), an intermediate filament that is canonically expressed in cerebral astrocytes, Slc6a13 (GAT-2), a major GABA transporter, and Gjb2 (Cx26), a CO2-sensitive gap junction for which defects have been implicated in congenital sensorineural hearing loss including KID syndrome as well as glial dysregulation31.