In addition, ex vivo analysis of splenic T cells in MC38 tumor bearing mice revealed that CD8+ IFN-γ producing effector T lymphocytes specific to MC38 tumor cell, but not to B16F10 tumor cells, were significantly augmented after intravenous injection of JCXH-211 but not of saline or blank LNP (Supplementary Fig. 5). The gene discussed is CD8A; the disease is neoplasm.