To further investigate the influence of protein synthesis and transport on the pairwise interactions of these three proteins, co-transfected tumor cells were incubated with CHX (inhibitor of protein synthesis) and brefeldin A (BFA, inhibitor of protein trafficking), with the finding that BFA significantly abrogated the interactions between STX18 and SEMA7A, FUT8 and STX18 and between FUT8 and SEMA7A. The gene discussed is SEMA7A; the disease is neoplasm.