To validate the results from the pharmacological inhibition of HIF-1α, we generated a genetic knockout of HIF-1α-coding gene (HIF1A) in S2-013 and HPAF-II pancreatic cancer cells by utilizing CRISPR/Cas9 technology (SI Appendix, Fig. S4 D and E). This evidence concerns the gene HIF1A and pancreatic neoplasm.