Six of the top ten DEGs that were up-regulated (ONECUT2, CES1, CD163, KCNN2, THBS1, and B3GALT1) and six of the top ten DEGs that were down-regulated (MYT1L, ASTN1, DLL3, MYO18B, POU4F1, and PCDH10) also had vital prognostic significance in the TCGA AML dataset (Fig. 6). This evidence concerns the gene ASTN1 and acute myeloid leukemia.