applied bioinformatics and biological data mining and found that NTNG1 was differentially expressed in adenocarcinoma and squamous patients, with low expression of NTNG1 in lung adenocarcinoma and high expression of NTNG1 in lung squamous cell carcinoma all being associated with poor prognosis of patients, indicating that NTNG1 is also involved in the occurrence and development of LC. This evidence concerns the gene NTNG1 and adenocarcinoma.