CD8A and chronic hepatitis B virus infection: We previously demonstrated that this strategy of cytokine analysis was reliable, since we showed a positive correlation between supernatant cytokine level, tested by ELISA, and cytokine mean fluorescence intensity after intracellular cytokine staining checked by flow cytometry on HBV-specific CD8+ T cell after 10-day Ag-specific cultures in HBeAg(−) chronic hepatitis B (6).