We implanted MC38-H2B-mApple tumor cells in IL12-eYFP reporter mice, which were treated with Pacific Blue-dextran as a macrophage-imaging agent to allow for the simultaneous visualization of tumor cells (mApple), TAMs (Pacific Blue) and TAM phenotype (co-expression of IL-12 and eYFP), respectively. The gene discussed is H2BC21; the disease is neoplasm.