PTPRC and neoplasm: Both dLNs and tumors were dissociated into single cell suspensions using an enzymatic cocktail as previous reported (26), followed by immunostaining and analyzed by flow cytometry to quantitate the percentages of FITC-positive CD11C+ total DCs, CD11C+/B220+ pDCs and CD11C+/B220- cDCs in dLNs and tumor tissues.