Figure 5 demonstrates the diversity in organoid organization. Texas Red phalloidin staining for F-actin demonstrated that some clusters had apical columnar organization (Figure 5A). Other spheroids were entirely cobblestone, exhibited a ring of cells on the surface of the sphere, or were unorganized (Figures 5B–D). On occasion, we had tumor cells migrate out from spheres (Figures 5E–N). These non-spheroid/differentiated cells could be epithelial as indicated through expression of E-cadherin (Figures 5I–K) or mesenchymal as indicated by vimentin expression (Figures 5L–N). The gene discussed is VIM; the disease is neoplasm.