Compared with those in WT and sh-NC groups, the expression levels of PI3K, p-Akt, p-mTOR, Notch1 and Hes1 proteins in the sh-PRDX6 group were significantly decreased (P < 0.05), and those of the unphosphorylated Akt and mTOR did not significantly change, suggesting that PRDX6 may participate in the regulation of the PI3K/Akt/mTOR signaling pathway and Notch signaling pathway in HCC (Fig. 6 and Table 3). The gene discussed is HES1; the disease is hepatocellular carcinoma.