At 48 h post-infection, an immunoblot revealed that only cells infected with OAdSA or OAdSA-4-1BBL showed Ad E1A expression, and using an antibody against murine 4-1BBL, we detected strong SA-4-1BBL production in cells infected with OAdSA-4-1BBL (Figure 3B). Here, DHTKD1 is linked to infection.