After further analysis using single-cell flow mass spectrometry (CyTOF) of the tumors of 4T1 orthotopic tumor-bearing mice, it was observed that ZD-E-1M was involved in the regulation of the tumor immune microenvironment (TIME), increasing the number of dendritic cells (DCs) and CD4-positive T cells but decreasing the number of myeloid-derived suppressor cells (MDSCs) and the abundance of LAG3 on various immune cells [74]. The gene discussed is CD4; the disease is neoplasm.