Nanoparticles showed reduced tumor growth compared to the growth in the control groups; tumor size rapidly increased to ~738 mm3 and ~674 mm3 for the mouse administered with PBS and EGFP mRNA PGDP nanoparticles, respectively, that are substantially larger than 288 mm3 for the group treated with PTEN-mRNA PGDP nanoparticles. The gene discussed is PTEN; the disease is neoplasm.