MA−24 significantly inhibited tumor growth compared to the vehicle (p < 0.0001) and positive control TH287 (tumor growth inhibition rate: 61.8% vs. 20.4%) (Figure 5a–d), and reduced lung metastasis (Figure 5e,f), indicating that MA−24, as an effective MTH1 inhibitor, possesses notable in vivo anti-tumor and anti-metastatic activity. Here, NUDT1 is linked to neoplasm.