Based on the mechanism of its protective effect on APAP-induced liver injury, it was shown that AEOV could promote the phosphorylation of AMPK, AKT, and GSK-3β protein expression, activate the AMPK/AKT/GSK-3β signaling pathway and inhibit Caspase-3 expression; it could also inhibit JNK phosphorylation, up-regulate Bcl-2 protein expression and down-regulate Bax and Caspase-9 protein expression, thus inhibiting apoptosis and exerting a protective effect on APAP-induced liver injury in mice. The gene discussed is GSK3B; the disease is injury.