We also found significantly lower DNA methylation levels of TNF-α in subjects with obesity and a WC ≥ 90 cm, TG concentrations ≥ 150 mg/dL, high blood pressure (systolic BP ≥ 130 mmHg or diastolic BP ≥ 85 mmHg), and FPG concentrations ≥ 110 mg/dL compared with those without MS (Table 2). Here, TNF is linked to obesity due to melanocortin 4 receptor deficiency.