EGFR and neoplasm: As indicated in both the in vivo and ex vivo images, the EGFR Affibody–IR700Dye conjugate clearly delineated the A253 tumor compared to the MCF7 tumor, suggesting that the EGFR Affibody–IR700Dye conjugate bound to the EGFR protein and imaged an EGFR-positive tumor (Figure 6a,b).