The results showed a significant decrease in Acetyl-CoA carboxylase 1 (Acc1) (Figure 4A) and sterol regulatory element binding protein (SREBP) cleavage activating protein (Scap) at the mRNA level in AML12 transfected with FGF21/HGFR compared to the control group in the NAFLD state, and an upregulation of hepatocyte growth factor (Hgf), and Albumin (Figure 4B). The gene discussed is FGF21; the disease is metabolic dysfunction-associated steatotic liver disease.