NF1 and plexiform neurofibroma: Six or more café-au-lait macules over 5 mm in diameter in prepubertal individuals or over 15 mm in postpubertal individualsFreckling in axillary of inguinal regionsTwo or more neurofibromas of any type or one plexiform neurofibromaOptic pathway gliomaTwo or more iris Lisch nodules, or two or more choroidal abnormalitiesSphenoid dysplasia, anterolateral bowing of the tibia, or pseudoarthrosis of long boneHeterozygous pathogenic NF1 variant with a variant allele fraction of 50%