The effects of the DIM-3,5 and DIM8-3,5 analogs on transactivation were determined using U87G human glioblastoma cells transfected with GAL4-NR4A1 (LBD) or GAL4-NR4A2 (LBD) and the UAS-luc reporter gene, which contains five tandem GAL4-DNA binding sites. The gene discussed is NR4A2; the disease is glioblastoma.