KEGG pathway analysis revealed significant enrichment of the targeted DE mRNAs in pathways such as mitophagy-animal, cell cycle, FoxO signaling pathway, insulin resistance, prostate cancer, microRNAs in cancer, circadian rhythm, TNF signaling pathway, spliceosome, kaposi sarcoma-associated herpesvirus infection, cellular senescence, one carbon pool by folate, viral carcinogenesis, hepatitis B, autophagy-animal, nucleocytoplasmic transport, human T-cell leukemia virus 1 infection, amyotrophic lateral sclerosis, JAK-STAT signaling pathway, and glioma (Figure 6D). The gene discussed is TNF; the disease is central nervous system cancer.