COL7A1 and recessive dystrophic epidermolysis bullosa: For a non-viral topical treatment of patients with Recessive Dystrophic Epidermolysis Bullosa (RDEB), a severe genetic skin disorder caused by mutations in the COL7A1 gene, RDEB skin cells were transfected with a minicircle expressing the large cDNA size of the COL7A1 gene (8.9-kb) to restore the expression and secretion of the structural protein-type-VII collagen (C7) at the dermal and epidermal junction [51].