Interestingly, we found that the treatment of mIMCD3 cells with TNF-α, which has been demonstrated to enhance cyst growth [43], increased the expression of SMYD3, suggesting a positive feedback loop of SMYD3/TNF-α/NF-κB/SMYD3, in a similar manner to SMYD2 [26]. Here, NFKB1 is linked to cyst.