MTHFD2 and neoplasm: This analysis confirmed the association of TYMS, TK1, SHMT2, MTHFD2, and DHFR expressions with the main prognosis markers (hypermutation, MSI, iCluster, expression subtype, and tumour stage) while MTHFD1, and not MTHFD2, was more strongly associated with methylation status (Figure 5B).