PSEN1 and Alzheimer disease: The 5xFAD model was generated by co-expressing five mutations associated with AD in APP/PS1 transgenic mice (Swedish (KM670/671NL), Florida (I716V), and London (V717I) variants in the APP gene, as well as M146L (A > C) and L286V mutations in the PSEN1 gene) [33,50].