Flow cytometry was used to analyze the expression of three proteins: cleaved NOTCH1 produced by pathway activation; HES1, a downstream target of the Notch signaling; and AIOLOS, a member of the Ikaros family known to interfere with the Notch pathway and found to be deregulated in B-CLL. The gene discussed is IKZF3; the disease is B-cell chronic lymphocytic leukemia.