Upon testing the distribution of different B-cell subsets in POAG patients, there was a highly significant increase in the frequencies of the DN B-cell subset (CD19+CD27−IgD−) and the naïve B-cell subset (CD19+CD27−lgD+) (p < 0.001) for all POAG patients, while a significant decrease in the unswitched memory B-cell subset (CD19+CD27+IgD+) (p < 0.001) was found in this group as compared with the control group. This evidence concerns the gene CD27 and open-angle glaucoma.