Furthermore, specific strains of F. prausnitzii (EB-FPDK9, EB-FPDK11, and EB-FPYYK1) can reduce the expression of Toll-Like Receptor 4 (TLR4), thereby lowering levels of pro-inflammatory cytokines (TNF-α, MCP-1, IL-6) in mice with NASH, subsequently improving inflammatory responses in NASH. The gene discussed is TNF; the disease is metabolic dysfunction-associated steatohepatitis.