Therefore, the NF-κB pathway could mediate ATF4 and SLC7A11 expression and in the condition of simultaneous GPX4 silencing could induce ferroptosis in glioblastoma cells; in fact, depletion of GPX4 alone is not sufficient to induce ferroptosis of glioblastoma cells and only GPX4 silencing causes a moderate cell proliferation blockage without altering SLC7A11 and ATF4 levels. The gene discussed is ATF4; the disease is glioblastoma.