The effect of DDB on tau and p-tau protein levels is shown in Figure 4; the results indicated that tau phosphorylation level was increased at Ser202/Thr205 (p-S202/p-T205), Ser396 (p-S396), and Ser404 (p-S404) sites in the hippocampal and cortex extracts of the 3 × Tg-AD mice comparing to the WT mice, whereas DDB treatment effectively attenuated increased expression of these p-tau protein levels. This evidence concerns the gene MAPT and Alzheimer disease.