While the focus is generally on TGF-β mediated Smad signaling, there is some involvement of EGF-mediated MAPK signaling to instigate migration; however, in A549 lung adenocarcinoma cells, EGF had no effect on the EMT marker’s MMP2 expression, and EGF was not responsible for significantly increasing the invasive potential of these cells, highlighting the importance of TGF-β to EMT and invasion as discussed [64]. This evidence concerns the gene TGFB1 and lung adenocarcinoma.