PKLR and Hemolytic anemia due to red cell pyruvate kinase deficiency: Various tests, including direct anti-globulin test, osmotic gradient ektacytometry [7, 8], PKLR sequencing, blood smear, and hemoglobin electrophoresis [9], ruled out other diagnoses such as hereditary spherocytosis, pyruvate kinase deficiency, autoimmune hemolytic diseases, glucose-6-phosphate dehydrogenase deficiency, thalassemia, and sickle-cell disease.