The dynamic changes in the top 5 representative genes (Acp5, Cd52, Ckb, S100a4, S100a6) that determine the fate of macrophages during the pseudotime process are shown in Fig. S24f, and GEPIA2 analysis showed that changes in the top 5 key genes can affect the survival time of tumor patients (Fig. S24g). The gene discussed is S100A4; the disease is neoplasm.