CD4 and type 1 diabetes mellitus: Consistently, our multi-parametric FACS profiling of 190,000 cells from of 10 stage 3 T1DM and 9 healthy samples (10,000 cells per sample) (Additional file 1: Fig. S4a-c; Methods) validated the depletion of CD4+ T cells in T1DM (p-value = 0.037, Additional file 1: Fig. S4d-e).