Furthermore, this study suggests that the seven mitophagy-signature genes (HSP90AA1, RPS27A, EEF2, EIF4A1, EIF2S1, HIF-1α, BNIP3) may serve not only as potential biomarkers for myocardial ischemia–reperfusion but also as potential targets for future research and Paving the way for novel therapeutic approaches targeting myocardial ischemia–reperfusion injury. The gene discussed is RPS27A; the disease is myocardial ischemia.