Based on previous knowledge regarding the potential roles of different metals during virus infection and nucleic acids metabolism, three metals were selected for studies on their effect on the activity of Nsp1: calcium, manganese, and magnesium (Chaturvedi and Shrivastava 2005; Iotti et al. 2020; Li et al. 2022; Berlansky et al. 2022). This evidence concerns the gene SH2D3A and viral infectious disease.