Lastly, we investigated the association of the RNA velocity differences with four well-known AD pathological traits: neuritic plaque (NP) and neuronal neurofibrillary tangle (NFT) counts based on histochemistry silver stain, and overall β-amyloid load (β-amyloid) and PHF tau tangle density (tangles) based on molecularly specific immunohistochemistry. The gene discussed is MAPT; the disease is Alzheimer disease.