Circulating S100A8/A9 levels were an independent HF predictor after adjusting for the same variables in the validation cohort (HR: 2.15 [95% CI: 1.79–2.58], P < 0.001), confirming S100A8/A9 as a robust HF predictor (Fig. 2d, e). The gene discussed is S100A8; the disease is hydrops fetalis.